The Evolution of Insect Resistance to Bt Crops

A group of scientists at the University of Arizona have this week published a paper in Nature Biotechnology on the evolution of resistance in insect pests populations to insecticidal proteins from Bacillus thuringiensis (Bt) that are produced by transgenic crops. Resistance is defined as the phenotype of an individual that gives the individual the ability to survive on a transgenic insecticidal plant from egg to adult and provide viable offspring. The team analysed field and laboratory data from seventy-seven studies of thirteen pest species in eighteen countries across five continents. Entomologist Bruce Tabashnik and colleagues found well documented cases of field-evolved resistance to Bt crops in five major pests as of 2010. 60% of these cases occurred in the U.S.A, where approximately half of the world’s Bt crop acreage is planted. In some cases, resistance to Bt evolved within as little as two to three years, whilst in other cases Bt crops have remained effective for more than 15 years. The research team aimed to better understand how quickly insect populations are evolving resistance to Bt crops and how this is occurring.

Workshop participants assess a range of fodder and cereal crops that can be used as “refugia”, fostering stem borers susceptible to the Bt toxin. In a longstanding partnership under the Insect Resistant Maize for Africa (IRMA) project , CIMMYT works with the Kenya Agricultural Research Institute (KARI) to offer farmers maize varieties that resist borers, which otherwise cause heavy losses (approximately 12% of Kenya’s annual maize crop). In addition to conventional breeding, one source of resistance in developing these varieties has been the soil bacterium Bacillus thuringiensis. A gene from this bacterium inserted into “Bt maize” causes it to produce a protein that is selectively toxic to certain borer species. However, mutant resistant borers unaffected by the toxin will flourish and eventually predominate, unless farmers use refugia to maintain a susceptible population. At this workshop in December 2005, sponsored by IRMA at KARI’s Kitale center, 50 participants—including researchers, extension workers, and farmers—learned about progress in the development of insect-resistant maize and the importance of refugia, evaluating numerous crops in the field for their potential as refugia. For more information, see CIMMYT's December 2005 e-news story "Bug Havens Keep Maize Pest-Proof," available online at: http://www.cimmyt.org/newsletter/86-2005/344-bug-havens-keep-maize-pest-proof.  Image © CIMMYT (CC-BY-NC-SA 2.0)

Women assess a range of fodder and cereal crops that can be used as “refugia for stem borers susceptible to the Bt toxin. In a longstanding partnership under the Insect Resistant Maize for Africa (IRMA) project , CIMMYT works with the Kenya Agricultural Research Institute (KARI) to offer farmers maize varieties that resist borers, which otherwise cause heavy losses (approximately 12% of Kenya’s annual maize crop). In addition to conventional breeding, one source of resistance in developing these varieties has been the soil bacterium Bacillus thuringiensis. A gene from this bacterium inserted into “Bt maize” causes it to produce a protein that is selectively toxic to certain borer species. However, resistant borers unaffected by the toxin will reproduce and eventually predominate, unless farmers use refugia to maintain a susceptible population. 
Image © CIMMYT (CC-BY-NC-SA 2.0)

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Cassava Brown Streak Disease threatens the “Rambo root” further north in Africa

Necrosis on cassava roots infected with Cassava Brown Streak Disease

Necrosis on cassava roots infected with Cassava Brown Streak Disease © IITA (CC BY-NC licence)

by MaryLucy Oronje and Willis Ndeda Ochilo

Cassava Brown Streak Disease (CBSD) is threatening gains made in the intensification of cassava production in Africa. Since my colleague, Abigail Rumsey, reported on the resurgence of this disease in East Africa, and spread to Central and Western Africa, there are now reports of it spreading to northern Africa, thereby threatening food security of more than 100 million people.

Cassava is one of the most important staple crops grown in tropical Africa. Its extraordinary ability to survive high temperatures and tolerate poor soils has led to it being called the “Rambo root”. It is an important source of calories in the tropics, coming third only to rice and maize, respectively. Additionally, cassava is the cheapest known source of starch grown by poor farmers – many of whom are women – often on marginal land.

The threat posed by CBSD is particularly alarming considering its symptoms are not easily recognizable to an untrained or inexperienced person, and worse still, its symptoms are often mistaken for the more familiar Cassava Mosaic Disease (CMD). In addition, rising temperatures and changes in the genetic makeup of whiteflies threatens to gun down the “Rambo root”.

Tertiary vein chlorosis of cassava leaves caused by CBSD

Tertiary vein chlorosis of cassava leaves caused by CBSD © IITA (CC BY-NC licence)

CBSD manifests itself on leaves as patches of yellow areas (chlorosis), on the stems as dark-brown streaks and spots, and dark brown hard rots can be seen on the roots when cut. In severe attack, there are radial constrictions and/or pits on the root surface.

There is no specific international research organization for cassava, unlike wheat, rice, potato and maize, and no private-sector interest. Recently, initiatives and projects on cassava diseases have been spearheaded by national research organizations, such as the International Institute for Tropical Agriculture (IITA) and some international NGOs. However, focus has been on the Cassava Mosaic Disease, at the expense of CBSD. However, there seems to be a change of heart (and rightly so) with players such as Cassava Regional Centre of Excellence (CRCoE) working towards solutions for this devastating disease. The CRCoE, established under the East Africa Agricultural Productivity Program (EAAPP) was funded by the World Bank.

Some of the recommended management practices include the use of improved disease-free planting material, scouting and uprooting diseased plants, crop rotation and keeping weed-free fields to reduce whitefly infestation. While there seems to be some semblance of activity towards addressing the challenge posed by CBSD, a lot of research and awareness campaigns still need to be done considering the aforementioned threats and the crucial role cassava plays in food security.

Source: http://www.ciatnews.cgiar.org/2013/05/06/waging-war-on-cassava-viruses-couldnt-come-sooner

Plantwise Photo Of The Month – June

Plant clinic in Machakos, Kenya

Plant clinic in Machakos, Kenya © CABI

Photo Of The Month for June shows a plant clinic during market day at a village near Machakos, 50 kilometers outside Kenya’s capital city Nairobi. The photo shows a farmer showing a crop sample to a plant doctor, who is using his knowledge and reference material on the table to diagnose what is wrong with the crop and provide management information to the farmer.  You can read more about plant clinics in Kenya here

Bees aid early detection of fireblight in orchards

The bees leave fireblight bacteria in the tube.

Bees from the orchard leave any fireblight bacteria in the tube through which they enter their hive © Rudolf Moosbeckhofer, AGES

Fireblight is a bacterial disease caused by the species Erwinia amylovora that affects fruit trees including apple and pear. It also affects other members of the rose family, including roses, crabapples and hawthorn. The disease can quickly spread through a plant, killing it within a few months, and can devastate entire orchards within a season. Fireblight is difficult to control; there are no chemicals that are effective once the disease has taken hold, and the spread can mainly only be slowed by pruning infected branches. Early detection of fireblight is therefore essential. Researchers at the Vienna University of Technology have devised a method to detect the disease, not on the fruit trees but on the bees that pollinate them. Read more of this post

Black Rot Disease Hits Uganda

A photograph of a cabbage leaf showing symptoms of black rot. Image by USDA Forest Service via Wikimedia Commons (CC-BY 3.0)

A photograph of a cabbage leaf showing symptoms of black rot. Image by USDA Forest Service via Wikimedia Commons (CC-BY 3.0)

Vegetable farmers in the Kayunga and Mukono districts of Uganda are reporting crop losses due to black rot disease. One farmer, Twaha Kahooza of Kyampisi village, Kayunga Sub-county, says he had planted four acres of cabbages and was expecting about Shs18m (about £4,500 or US$7,000) from the harvest, however he only managed to get Shs5m (about £1,200 or US$2,000).

Black rot is caused by the bacterium Xanthomonas campestris pv. campestris and is one of the most destructive diseases of cabbage and other crucifers such as  broccoli, brussels sprouts, Chinese cabbage, collards, kohlrabi and mustard. The disease is usually most prevalent in low lying areas where plants remain wet for long periods. The disease is characterized by a yellow V-shaped lesion at the leaf margin which turns brown as the leaf area expands. The disease can also affect seedlings and can enter the plant through insect feeding or injury to the plant. Management of black rot in crucifers includes obtaining certified, pathogen free seed, ensuring there is enough space between plants and crop rotation.To read more about black rot and black rot management visit factsheets on the Plantwise Knowledge Bank.  

To read a Plantwise Factsheet for Farmers written in Uganda click here. 

To find out more about Plantwise plant clinics running in Uganda, click here

References:

‘Farmers count losses over black rot disease in cabbage’, Fred Muzaale, April 2013, Daily Monitor 

Plantwise Photo Of The Month- May

Antonio Limbau, Deputy Minister for Agriculture, Mozambique, speaking at the G8 Open Data for Agriculture Conference

Antonio Limbau, Deputy Minister for Agriculture, Mozambique, speaking at the G8 Open Data for Agriculture Conference (CABI Image)

This photo shows Antonio Limbau, the Deputy Minister for Agriculture of Mozambique speaking on the implications of agricultural open data for developing countries at the G8 Open Data for Agriculture conference on Monday in Washington, D.C. 

Governments in developed countries are working hard to make agriculture data open for others and accessible to farmers.  The talk focused on the implications of open data,  potentially a significant resource for developing countries working to help poor farmers increase their productivity. As well as Antonio Limbau, other speakers included Hirano Katsumi from the Area Studies Center, Japan, Stanley Wood from the Bill and Melinda Gates Foundation and Sean Krepp from the Grameen AppLab, Uganda. 

To read more about this conference visit the website and have a look at some of our other blog posts on the event

 

Top tweets from G8 Open Data for Agriculture – Day 2

Plantwise hosted an exhibit stand during the G8 conference, giving demos of the Knowledge Bank. Credit: Patricia Neenan © CABI

Plantwise hosted an exhibit stand during the G8 conference, giving demos of the Knowledge Bank. Credit: Patricia Neenan © CABI

With a successful first day at the G8 conference wrapped up, and hundreds of tweets posted with ideas for how open data can contribute to increased food security, Day 2 kicked off with Dr Kathryn Sullivan from NOAA welcoming the delegates, and a session on ‘What Does Open Data Look Like?’ chaired by Prof Tim Benton, who has guest blogged for Plantwise before. Join in the discussion on Twitter using #OpenAgData and #Plantwise, and follow us on Facebook for more news. Read more of this post

European Union Vote To Restrict Neonicotinoid Insecticide Use

Do you think the EU is right to ban neonicotinoids? Photo of a honeybee via Flickr (Brad Smith, CC-BY-NC-2.0)

Do you think the EU is right to ban neonicotinoids? Photo of a honeybee via Flickr (Brad Smith, CC-BY-NC-2.0)

After a European Commission vote yesterday (Monday 29th April 2013), Europe will enforce the world’s first continent-wide ban on neonicotinoid insecticides after concerns about their non-target impact on bee pollinators.

The vote by the 27 European Union member states on whether to suspend the use of neonicotinoid insecticides was supported by 15 nations. The UK did not support the ban.

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Top tweets from G8 Open Data for Agriculture – Day 1

Agriculture Secretary Tom Vilsack and Agriculture Under Secretary Dr. Catherine Woteki at the G-8 International Conference on Open Data for Agriculture

US Agriculture Secretary Tom Vilsack and Agriculture Under Secretary Dr. Catherine Woteki at the G-8 International Conference on Open Data for Agriculture. Credit: USDAgov (CC BY 2.0 license)

Delegates from around the world are convening in Washington D.C. this week at the G8 conference on Open Data for Agriculture. The goal of the conference is to: “Obtain commitment and action from nations and relevant stakeholders to promote policies and invest in projects that open access to publicly funded global agriculturally relevant data streams, making such data readily accessible to users in Africa and world-wide, and ultimately supporting a sustainable increase in food security in developed and developing countries”. Plantwise is exhibiting at the conference, and we are collecting some of the top tweets here on our blog. You can watch the whole conference live on the web here. Join in the discussion on Twitter using #OpenAgData and #Plantwise, and follow us on Facebook for more news. Read more of this post

Open Letter on Open Data from CABI’s CEO to G8 Delegates

Check out the latest message from CABI’s CEO on revitalising food security challenge through open data. He and other Plantwise reps are reporting live from DC for the G8 Conference on Open Data for Agriculture today and tomorrow. Download pdf of his letter here. Read more of this post

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